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The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and <t>CD4</t> + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.
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The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and <t>CD4</t> + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.
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The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and <t>CD4</t> + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.
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The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and <t>CD4</t> + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.
Magcellecttm Human Cd4 T Cell Isolation Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and CD4 + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.

Journal: Advanced Science

Article Title: Restoring Tumor Cell Immunogenicity Through Ion‐Assisted p53 mRNA Domestication for Enhanced In Situ Cancer Vaccination Effect

doi: 10.1002/advs.202500825

Figure Lengend Snippet: The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and CD4 + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.

Article Snippet: Mouse CD8 + T Cell Isolation Kit (CS103‐01) and Mouse CD4 + T Cell Isolation Kit (CS102‐01) (Nanjing Vazyme Biotech Co., Ltd); Gel Rapid Extraction Kit (JiangSu CoWin Biotech (CWBIO)); StarMarker 1kb Ladder Plus (M015) and StarStain Red Plus10000× (E110) (GenStar (Beijing, China)); IL‐10 ELISA Kit (CSB‐E04594m‐IS, CUSABIO, https://www.cusabio.com/ ); Sparkjade ECL super (Shandong Sparkjade Biotechnology Co., Ltd.); T7 High Yield RNA Transcription kit, RNase inhibitor and ATP (Novoprotein Scientific (China)); Aminoallyl‐UTP (MedBio Pharmaceutical Technology Inc); MDM2 antibody (bs‐20790R) (Beijing Biosscn Biotechnology Co., Ltd).Anti‐p53 antibody [PAb 240] (ab26), anti‐MHC class I antibody [R1‐9.6] (ab281903), anti‐CD80 antibody (ab254579), anti‐PD‐L1 antibody [EPR20529] (ab213480), anti‐GAPDH antibody (ab8245), anti‐HMGB1 antibody (ab18256), anti‐Calreticulin antibody [EPR3924] (ab92516), goat Anti‐Mouse IgG H&L (HRP) (ab205719), goat Anti‐Rabbit IgG H&L (HRP) (ab205718), anti‐CD41 antibody [EPR17876] (ab181582) and anti‐Hemoglobin subunit alpha antibody [EPR3608] (ab92492) were ordered from Abcam (Cambridge, UK).

Techniques: Mutagenesis, Immunofluorescence, Expressing, Flow Cytometry