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Image Search Results
Journal: Advanced Science
Article Title: Restoring Tumor Cell Immunogenicity Through Ion‐Assisted p53 mRNA Domestication for Enhanced In Situ Cancer Vaccination Effect
doi: 10.1002/advs.202500825
Figure Lengend Snippet: The antitumor effects of PRIZE+NIR in inhibiting P53 mutant tumors. a) Schematic illustration of PRIZE+NIR‐triggered cancer immunotherapy in a MC38 tumor model. b) Tumor growth curves of tumors (n = 8 biologically independent samples). c) Representative immunofluorescence images showing the expression of CRT, HMGB1 in tumors treated by the indicated formulations in combination with NIR. d) Immunofluorescence analysis of CD8 + GZMB + T cells and CD4 + Foxp3 + T cells tumors of different groups. e,f) Flow cytometry and quantitative flow cytometry data of infiltrated CD3 + CD8 + T cells and CD3 + CD4 + T cells in tumors of mice in different groups (n = 3 biologically independent samples). g,h) Flow cytometry and quantification of expression levels of CD8 + GZMB + T cells and CD8 + IFN‐γ + T cells in tumors in different groups (n = 3 biologically independent samples). The representative gating strategies are provided in Figure (Supporting Information). i) Overall survival rate of C57BL/6 mice with MC38 tumor in in different groups (n = 8 biologically independent samples). Data are presented as mean ± SD. Statistical analyses were done using one‐way ANOVA with Tukey's multiple comparisons test and correction. *P <0.05, * *P <0.01, ** *P <0.001, *** *P <0.0001, ns, not significant.
Article Snippet: Mouse CD8 + T Cell Isolation Kit (CS103‐01) and
Techniques: Mutagenesis, Immunofluorescence, Expressing, Flow Cytometry